84-2412-93　［取扱停止］WIP (WAS/WASL-interacting Protein Family Member 1, Protein PRPL-2, Wiskott-Aldrich Syndrome Protein-interacting Protein, WASP-interacting Protein, WIPF1, WASPIP, WIP) 50ul　226696

特徴

• Mutations in the Wiskott-Aldrich syndrome protein (WASP) often result in immunodeficiency due to abnormal T cell and B cell activation.
• The 503 amino acid WAS-interacting protein (WIP) contains a number of domains implicated in Actin-binding and several putative SRC homology-binding domains.
• The first 100 amino acids of WASP interact with amino acids 377-503 of WIP, and the majority of pathogenic mutations associated with WAS occur within the first 100 amino acids of WASP.
• The gene encoding human WIP maps to chromosome 2q31.1.
• Overexpression of WIP in the human B cell line BJAB increases F-Actin content and cerebriform projections.
• While both WIP and Vav cooperate in the regulation of NF-AT/AP-1 gene transcription, the WIP-WASP complex is required for activation of NF-AT/AP-1 necessary for proper T cell function.
• A dysfunctional WIP-WASP complex may be implicated in the immunodeficient phenotype in WAS.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.