Tryptophanyl-tRNA synthetases are essential enzymes that catalyze the aminoacylation of tRNA(trp) with tryptophan, an essential function of the protein synthesis machinery of the cell.
Two forms of tryptophanyl-tRNA synthetase exist: a cytoplasmic form, named TrpRS (also known as WARS), and a mitochondrial form, named WARS2.
In normal cells, human TrpRS exists as a full length form and as a truncated form designated mini TrpRS, which is produced by alternative splicing.
Expression of mini TrpRs is highly stimulated in human cells by the addition of IFN-gamma.
Although both human full-length TrpRS and mini TrpRS are enzymatically active in aminoacylation, they differ in angiostatic activity.
The gene encoding human TrpRS maps to chromosome 14q32.31 and the gene encoding human WARS2 maps to chromosome 1p13.3-p13.1.
The first 18 amino acids of WARS2 constitute the mitochondrial localization signal sequence.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of WARS. Species Crossreactivity: Human, mouse, rat
