84-2412-52　［取扱停止］VPAC1 (VIPR1, VIPR1, PACAP-R2, VIP-R-1, Vasoactive intestinal Polypeptide Receptor 1) 100ul　226674

特徴

• The vasoactive intestinal peptide (VIP) and the pituitary adenylate cylaseactivating polypeptide (PACAP) belong to a superfamily of peptide hormones that include glucagon, secretin and growth hormone releasing hormone.
• The effects of VIP and PACAP are mediated by three G-protein coupled receptors, VPAC1, VPAC2 and the PACAP receptor (also designated PAC1-R).
• The VPAC receptors have equal affinities for VIP and PACAP, which stimulate the activation of adenylyl cyclase.
• Both VPAC1, a 47kD protein, and VPAC2, a 65kD protein, are abundantly expressed in brain and T cells, where they modulate neuronal differentiation and T cell activation, respectively.
• The PACAP receptor is a seven transmembrane protein that produces at least eight isoforms by alternative splicing.
• Each isoform is associated with a specific signaling pathway and a specific expression pattern.
• The PACAP receptor, which is thought to play an integral role in brain development, preferentially binds PACAP in order to stimulate a cAMP-protein kinase A signaling pathway.
• Applications:Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.