Angiogenesis is mainly regulated by the balance of several different pro-angiogenic stimulators, such as vascular endothelial growth factor (VEGF) and a diverse group of endogenous inhibitors that are extrinsic to endothelial cells.
Vasohibin is a secreted protein that is induced by a specific, self-regulating, feedback inhibition response to inhibit angiogenesis in an autocrine manner.
It inhibits proliferation, migration, and network formation by endothelial cells.
This function is specific for endothelial cells as it does not affect migration in other cell types.
Vasohibin is primarily expressed in endothelial of the brain and placental tissues with highest abundance in fetal organs.
VEGF and fibroblast growth factor 2 upregulate the expression of Vasohobin.
In vitro, Vasohibin does not affect cancer cell proliferation, but does inhibit tumor growth and angiogenesis.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Vasohibin. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 268-324 of Human Vasohibin.
