The ubiquitin pathway involves three sequential enzymatic steps that facilitate the conjugation of ubiquitin and ubiquitin-like molecules to specific protein substrates.
Through the use of a wide range of enzymes that can add or remove ubiquitin, the ubiquitin pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression.
USP35 (ubiquitin specific peptidase 35) is a 1,017 amino acid protein that belongs to the peptidase C19 family.
Expressed in pancreas, testis and skeletal muscle, USP35 functions to catalyze the conversion of a ubiquitin C-terminal thioester and water to free ubiquitin and a thiol, a reaction that may play a role in signaling events throughout the cell.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of USP35. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human USP35.
