The ubiquitously expressed cellular upstream stimulatory factor (USF) consists of 43kDa (USF-1) and 44kDa (USF-2) polypeptides which independently exhibit site-specific DNA binding and are members of the c-Myc-related family of regulatory factors containing helix-loop-helix domains.
USF also contains a leucine repeat that is required for efficient DNA binding.
USF was originally identified as an upstream stimulatory factor that binds the core sequence CACGTG in the adenovirus late promoter.
These findings, together with the demonstration of cooperative interaction between USF and the initiator-binding protein TFII-I, raise the possibility of a more general involvement of USF in transcriptional regulation.
While expression of both USF-1 and USF-2 species is ubiquitous, different ratios of USF homo- and hetero-dimers are found in different cell types.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 15mM sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of USF2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 196-244 of Human USF2.
