UDP-GlcDH (also called P-glucose 6-dehydrogenase, UGDH or UDPGDH) is a member of the UDP-glucose/GDP-mannose dehydrogenase family.
UDP-GlcDH converts UDP-glucose to UDP-glucuronic acid, which is a crucial component in the biosynthesis of the glycosaminoglycans, hyaluronan, heparan sulfate and chondroitin sulfate.
Found as common components of the extracellular matrix, these glycosaminoglycans are significant in signal transduction, cell migration, cancer growth and cancer metastasis.
UDP-glucuronic acid (UDP-GlcA) is needed in the liver for the excretion of toxic compounds.
UDP-GlcDH is a ubiquitously expressed protein most abundant in the liver.
The protein structure of UDP-GlcDH was first analyzed in cow liver and found to be a homohexamer.
This structure is well conserved between species and phyla with an overall 97% sequence identity shared between different species of mammals.
Research indicates that UDP-GlcDH expression is upregulated by TGFbeta and downregulated by hypoxia.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of UGDH / UDP. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 411-460 of Human UGDH.
