Two major regulatory peptides were originally isolated from fish urophysial extracts, Urotensin I and II .
In both frog and human, the Urotensin II sequence is located at the carboxy-terminal position of the precursor .
Human Urotensin II is composed of only 11 amino acid residues, while fish and frog Urotensin II possess 12 and 13 amino acid residues, respectively.
The cyclic region of Urotensin II, which is responsible for the biological activity of the peptide, has been fully conserved from fish to human.
However, several substitutions have occurred in the amino-terminal region of the molecule .
A human G protein-coupled receptor, GPR14, is the Urotensin II receptor .
Human Urotensin II is found within both vascular and cardiac tissue, including coronary atheroma, and effectively constricts isolated arteries from nonhuman primates.
Urotensin II may act as an autocrine and/or paracrine hormone rather than as a circulating hormone, by playing an important role in the development of ventricular hypertrophy induced by chronic hypoxia.
Applications:Suitable for use in Western Blot and Immunohistochemistry/Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry (Immunofluorescence): 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human UCN2.
