UCHL1 is a single chain transmembraneous glycoprotein which represents the low molecular weight isoform of the Leucocyte Common Antigen (LCA).
It is expressed on most thymocytes, about 45% of peripheral blood T cells, virtually all T cells in skin reactive infiltrates, and the majority of T cell malignancies.
It is also found on a subset of B cells and on exceptional B cell lymphomas.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot:1:500-1:1000Immunohistochemistry:1:50-1:200Immunoprecipitation:Immunoprecipitation of the Testis tissue lysate of Rat using UCHL1 pAb (Sepharose Bead Conjugate) (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)(lane 4 and lane 5) .Lane 1 is 30% input.
The western blot was probed using UCHL1 pAb .“↑”(supernatant); “↓”(deposition)Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of UCHL1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 171-220 of Human UCHL1.
