Ubiquitin (Ub) is among the most phylogenetically conserved proteins known.
The primary function of ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome.
This small, 76 amino acid protein can be covalently attached to cellular proteins via an isopeptide linkage between the carboxy terminal group of ubiquitin and lysine amino groups on the acceptor protein.
For proteolysis to occur, ubiquitin oligomers must be assembled.
Ubiquitin chains on proteolytic substrates are commonly found to have an isopeptide bridge between Lysine 48 of one ubiquitin molecule and the carboxy-terminus of a neighboring ubiquitin molecule.
Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IkBa and p27.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:1000Immunohistochemistry (Paraffin): 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, pH 7.2, 0.05% sodium azide.
Specificity：Recognizes human biquitin, polyubiquitin and ubiquitinated proteins. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 20-60 of human Ubiquitin (P62988).
