The transcription of ribosomal RNA genes by RNA polymerase I (Pol I) is tightly coordinated with the growth state of the cell.
In addition to Pol I, transcription of ribosomal genes requires the trans-activating factor UBF (upstream binding factor).
UBF functions by binding to DNA elements within the RNA gene promoter and enhancer regions and directly associating with Pol I, tethering it to the promoter complex.
Two UBF proteins, of 97kDa and 94 kDa, arise from the same gene as a result of alternative mRNA splicings.
UBF activity is regulated by several dependent casein kinase II phosphorylates at the carboxy terminus of UBF on serine residues.
The retinoblastoma susceptibility gene product, Rb, when not bound to E2F family members, inhibits UBF activity.
Expression of RNA may also be negatively regulated by the 70kDa and 86kDa Ku antigens.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of UBF-1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 500-550 of Human UBF-1.
