E3 ubiquitin ligases are a large family of proteins that are engaged in the regulation of the turnover and activity of many target proteins and are classified into three major structurally distinct types: N-end rule E3s, E3s containing the HECT (homology to E6AP C-Terminus) domain and E3s with the RING finger.
UBE3C (ubiquitin protein ligase E3C), is a 1083 amino acid nuclear protein that contains one HECT domain and one IQ domain.
Existing as three isoforms due to alternative splicing events, UBE3C is highly expressed in skeletal muscle and expressed at much lower levels in kidney and pancreas.
UBE3C is a E3 ubiquitin-protein ligase that accepts ubiquitin from UBE2D1 (ubiquitin-conjugating enzyme E2 D1) in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
UBE3C can target itself for ubiquitination in vitro and may promote its own degradation in vivo.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of UBE3C. Species Crossreactivity: Human
