Uev1A, also designated Uev1, UBE2V1 for Ubiquitin-conjugating enzyme E2 variant 1 and CROC1, shows sequence similarity to other ubiquitin-conjugating enzymes, but lacks the conserved cysteine residue critical for their catalytic activity .
Therefore, Uev1A does not have ubiquitin-conjugating activity in vitro .
However, constitutive expression of exogenous Uev1A in colon carcinoma cells inhibits their capacity to differentiate upon confluence.
Studies on recombinant Uev1A show that it localizes to the nucleus, excluding the nucleolar regions .
Uev1A functions with TRAF6, a RING domain protein, to catalyze the synthesis of unique polyubiquitin chains linked through lysine-63 of ubiquitin .
The gene encoding Uev1A maps to human chromosome 20q13.2 .
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of UBE2V1. Species Crossreactivity: Human, mouse
