Rub1 shares 53% homology with ubiquitin and requires activation via the E2 proteins, including Ula1, Uba3 and Ubc12, in order to conjugate to substrates directed to different proteolytic systems.
UBE2D2 catalyzes ubiquitination of IkBa in a phosphorylation and SCFB-TRCP dependent manner.
In this particular reaction, E1 first transfers ubiquitin to the E2 component UBE2D2, and UBE2D2 then associates with E3 ligase, which conjugates the poly-ubiquitin chain on a target protein.
In this fashion, the chain tags the IkBa for degradation by a proteasome thus lifting the inhibitory effect of IkBa on NFkB and allowing NFkB to enter the nucleus.
Applications:Suitable for use in Western Blot, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunocytochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of UBE2D2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 102-147 of Human UBE2D2.
