84-2409-71　［取扱停止］TWIST1 (Twist-Related Protein 1, Class A basic Helix-Loop-Helix Protein 38, bHLHa38, H-twist, BHLHA38, TWIST) 100ul　226530

特徴

• Members of the myogenic determination family are basic helix-loop-helix (bHLH) proteins that can be separated into two classes.
• Class A proteins include the ubiquitously expressed E-box binding factors E12/E47, ITF2 and HEB (BETA1 or HTF4).
• Class B proteins such as MyoD, myogenin and NeuroD (BETA2) are transiently expressed and exhibit a much more limited tissue distribution.
• Class A proteins heterodimerize with class B proteins to activate DNA transcription.
• Working in opposition to these positively acting factors are a specialized group of proteins that function as dominant negative regulators.
• Muscle tissue is derived from a subset of cells originating from the embryonic mesoderm.
• The novel basic helix-loop-helix (bHLH) transcription factor twist is a putative regulator of mesodermal differentiation and myogenesis.
• Twist is expressed throughout the epithelial somite but not in the myotome.
• Twist requires dimerization with the E proteins and inhibits myogenic regulatory factors.
• It has been implicated as regulator of the temporal and spatial formation of myotomes.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.