Hyperpolarization-activated, cyclic nucleotide-binding channels (HCN) are voltage-gated cation channels that are activated by direct binding of intracellular cyclic nucleotides.
The HCN family consists of four members (HCN1–4), each with a core transmembrane segment domain and a carboxy-terminal 120 amino-acid cyclic nucleotide-binding domain motif.
HCN channels are expressed in the brain, heart, thalamus and testis.
The pacemaker properties of HCN channels contribute to spontaneous rhythmic activity in the brain and heart.
The genes encoding human HCN1 and HCN2 map to chromosomes 5 and 19p13.3, respectively.
The genes encoding HCN3 and HCN4 map to chromosomes 1q21.3 and 15q24-q25, respectively.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of HCN2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 490-541 of Human HCN2.
