84-2409-52　［取扱停止］Tubulin y (Tubulin gamma-1 Chain, Gamma-1-Tubulin, Gamma-Tubulin Complex Component 1, GCP-1, TUBG1, TUBG, Tubulin y1, Tubulin-y1, Tubuliny1) 50ul　226521

特徴

• TUB4, the gene for Saccharomyces cerevisiae y-Tubulin, encodes a 473 amino acid structural protein that localizes to the spindle pole body.
• y Tubulin, an essential protein for cell growth, organizes microtubule arrays in the nucleus and cytoplasm.
• y Tubulin-depleted cells fail to form functional spindles and arrest during nuclear division.
• y Tubulin associates with spindle body components Spc97 and Spc98 to form the y Tubulin complex.
• The budding yeast y Tubulin complex contains one molecule each of Spc97 and Spc98 and two molecules of y Tubulin.
• In the SPB, Spc110 binds Spc97 and Spc98 of the y Tubulin complex.
• 2D gel analysis indicates five isoforms of y Tubulin.
• The phosphorylation of y Tubulin at Tyr 445 plays a regulatory role in microtubule formation.
• The incidence rate for this phosphorylation event peaks during G1.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.