Thymic stromal lymphopoietin (TSLP) is a novel member of the hemopoietic cytokine family that promotes the development of B cells and shares overlapping activity with IL-7.
The gene encoding murine TSLP maps to chromosome 18 and its human homologue is expressed in several tissues, including heart, liver and prostate.
TSLP mediates its function by binding to a receptor complex.
TSLP first binds with low affinity to a TSLP-specific chain designated TSLPR, and then forms a high affinity complex with the IL-7Ralpha subunit, which explains the overlapping biological properties between TSLP and IL-7.
Both TSLP and IL-7 induce phosphorylation of the transcription factor Stat5, but unlike IL-7, TSLP-mediated signaling does not activate the JAKs.
TSLP prevents apoptosis and stimulates the proliferation of myeloid cells, which is supported by the coexpression of TSLPR and IL-7RRalpha on monocytes and dendritic cells.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of TSLP. Species Crossreactivity: Human
