In the small intestine, each isoform is cleaved by Enterokinase into its active form, Trypsin-1, Trypsin-2 and Trypsin-3, respectively.
All trypsins are members of the serine protease trypsin family.
The activated trypsins go on to activate other protease zymogens and play a role in the autoactivation of trypsinogens.
This suggests an important role for trypsins in digestion.
Mutations in the gene encoding Trypsin-1 that stimulate its activity are associated with autosomal dominant hereditary pancreatitis (HCP), also known as chronic pancreatitis (CP), a disease characterized by persistent, severe abdominal pain due to calcifications of the parenchyma, pancreatic stones, cysts and pancreatic head enlargement.
Trypsin-3 is expressed in the brain in addition to the pancreas.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Trypsin-3. Species Crossreactivity: Human
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to amino acids 150-200 of Human Trypsin-3.
