TRIM5a is a 493 amino acid member of the large tripartite motif protein (TRIM) family.
TRIM proteins are composed of three zinc-binding domains, a RING, a B-box 2 and a coiled-coil domain, and they use homomultimerization to identify different cell compartments.
Some TRIM proteins, such as TRIM5a, also possess a carboxy-terminal B30.2 (SPRY) domain and localize to the cytoplasm.
TRIM5a mediates innate intracellular retroviral resistance, which is dependent on its carboxy-terminal domain.
The three variable regions of the B30.2 domain form loops on one side of the B30.2 core structure of TRIM5a which may form a binding surface for the virus.
TRIM5a trimerization plays a major role in its affinity for the retroviral capsid, and in its ability to inhibit virus infection.
The linker region between the coiled-coil and B30.2 domains of TRIM5a is required for this trimerization.
TRIM5a blocks infection after the virus has entered the cell.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of TRIM5. Species Crossreactivity: Human
