TRB-3 (tribbles 3), also called NIPK (neuronal cell death-inducible protein kinase) disrupts insulin signaling by binding directly to Akt kinases and blocking their activation.
TRB3 binds to ATF4, inhibiting its transcriptional activation activity, and regulates activation of MAP kinases.
In the liver, TRB-3 is a target for PPAR-alpha.
Amounts of TRB3 RNA and protein are higher in livers of diabetic mice compared with those in wildtype mice.
TRB3 contributes to insulin resistance in individuals with susceptibility to type II diabetes.
Highest expression of TRB-3 is in liver, pancreas, peripheral blood leukocytes and bone marrow.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of TRIB3. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human TRIB3.
