Thyrotrophin-releasing hormone (TRH) is a hypothalamic tripeptide that stimulates, via its receptor in the anterior pituitary gland, the release of thyrotrophin (TSH) and prolactin.
The TRH receptors, TRH-R1 and TRH-R2, are G protein-coupled proteins containing seven transmembrane domains and other conserved regions.
In rat, two isoforms exist, TRH-R (412) and TRH-R (387), that differ at their carboxy termini.
TRH receptors are distributed throughout the central and peripheral nervous systems and are present in a variety of tissues.
TRH-R2 displays 50% homology to TRH-R1 and is more restricted to the central nervous system than TRH-R1.
Mutation in the TRH receptor gene is associated with isolated central hypothyroidism, a rare disorder characterized by insufficient TSH secretion resulting in low levels of thyroid hormones.
Applications:Suitable for use in Immunofluorescence, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of TRH-R1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 250-300 of Human TRH-R1.
