Telomeric repeat binding factor 1 (TERF1, PIN2, TRF1, TRBF1) and 2 (TERF2, TRF2, TRBF2) are present at telomeres throughout the cell cycle where they regulate telomerase by acting in cis to limit the elongation of individual chromosome ends.
Telomerase adds hexameric repeats of 5'-TTAGGG-3' to the ends of chromosomal DNA.
This telomerase enzyme plays an influential role in cellular immortalization and cellular senescence.
TRF1 negatively regulates telomere elongation, while TRF2 protects the chromosome ends by inhibiting end-to-end fusions.
Down-regulation of TRF expression in tumor cells may contribute to cell immortalization and malignant progression.
TRF1 has an acidic N-terminus while TRF2 has a basic N-terminus.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 15mM sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of TRF1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 200-250 of Human TRF1.
