84-2408-01　［取扱停止］TRAP100 (Mediator of RNA Polymerase II Transcription Subunit 24, Activator-recruited Cofactor 100kD Component, ARC100, Cofactor required for Sp1 Transcriptional activation Subunit 4, CRSP Complex Subunit 4, Mediator Complex Subunit 24, Thyroid Hormone Receptor-Associated Protein 4, Thyroid Hormone Receptor-Associated Protein Complex 100kD Component, Trap100, hTRAP ...　226440

特徴

• In mammalian cells, transcription is regulated in part by high molecular weight coactivating complexes that mediate signaling between transcriptional activators and initiation factors.
• These complexes include the thyroid hormone receptor-associated protein (TRAP) complex, which interacts with thyroid receptors (TR), vitamin D receptors and other steroid receptors to facilitate hormone induced transcriptional activation.
• The TRAP complex consists of numerous proteins ranging in size from 20 to 240 kDa, including TRAP95, TRAP100, TRAP150, TRAP220 and TRAP230, and they are characterized by the presence of a nuclear receptor recognition motif, which mediates the ligand-dependent binding of TRAP proteins to the nuclear receptors.
• TRAP220 and TRAP100 are widely expressed and most abundantly detected in skeletal muscle, heart and placenta.
• Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.