84-2407-95　［取扱停止］TRalpha (Thyroid Hormone Receptor alpha, Nuclear Receptor Subfamily 1 group A Member 1, V-erbA-Related Protein 7, EAR-7, c-erbA-1, c-erbA-alpha, THRA, EAR7, ERBA1, NR1A1, THRA1, THRA2) 100ul　226437

特徴

• Thyroid hormone nuclear receptors (TRs) are ligand-dependent transcription factors which regulate growth, differentiation and development and represent members of the steroid/retinoic acid superfamily.
• The two genes encoding TRs identified to date, TRalpha and TRbeta, have been mapped to human chromosomes 17 and 3, respectively.
• TRs bind to thyroid hormone response elements (TREs) with half-site binding motifs in the orientation of palindromes, direct repeats or inverted palindromes.
• The affinities of binding are both variable and influenced differentially by 3,5,3'-triiodo-L-thyronine (T3).
• Transcriptional regulation by TRs is also modulated by heterodimerization with TR nuclear accessory proteins, the most extensively characterized of which are the retinoid X receptors (RXRalpha, RXRbeta and RXRγ).
• To a certain extent, this activity is regulated by differential phosphorylation of TRs.
• Thus, not only are the biological activities of TRs regulated by heterodimerization with RXRs, but in addition, the gene regulatory activities of TRs are linked to other hormonal pathways.
• TRalpha1 can display both a nuclear and undefined cytoplasmic location, and is the only TR that is imported into the mitochondrial matrix.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.