Tumor necrosis factor receptor-associated factor (TRAF) protein family members are critically involved in pathways triggered by tumor necrosis factor (TNF) receptors and Toll/interleukin-1 receptor (TIR)-containing receptors.
TRAF7 contains an N-terminal RING and zinc finger domain and localizes to the cytoplasm and nucleus of cells in the M1 phase.
TRAF7 activates IKKs-I˚Balpha and MKK3/6-p38 pathways which stimulate Toll-like receptor 2 (TLR2) signaling.
TLR2 activates innate immunity, induces development of the acquired immunity, and also leads to the harmful inflammatory responses that can occur with infectious diseases.
TRAF7 has E3 ubiquitin ligase activity, and it binds to the DNA-binding domain of the oncogenic c-Myb, via the WD40 repeats, and stimulates its sumoylation.
This forces c-Myb to stay in the cytosol rather than the nucleus and thereby inhibits its activity.
TRAF7 also stimulates MEKK3-mediated AP1 and CHOP activation and induces apoptosis.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of TRAF7. Species Crossreactivity: Human, mouse, rat
