Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1.
Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors.
Several tryptase genes are clustered on chromosome 16p13.3.
These genes are characterized by several distinct features.
They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability.
These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence.
This feature is characteristic of tryptases but is unusual in other genes.
The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1.
Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate.
Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders.
Applications:Suitable for use in Western Blot and Immunofluorescence.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:2000Immunofluorescence: 1:20-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, 50% glycerol
Specificity：Recognizes endogenous levels of human TPSAB1. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo
Immunogen：Full length recombinant protein corresponding to human TPSAB1
Shelf Life：1year
Swiss Prot Number：Q15661
EU Commodity Code：30021010
商品名：TPSAB1 (alpha II, EC 3.4.21.59, Lung tryptase, Mast Cell alpha II tryptase, Mast Cell beta I tryptase, Mast Cell Protease 7, Mast Cell Protease II, MCP 7, MMCP 7, Pituitary tryptase, Skin tryptase, TPS 1, TPS1, TPS2, TPSAB1, TPSAB1 Protein, TPSB1, Tryptase 1, Tryptase alpha 1, tryptase alpha I included, Tryptase alpha II, tryptase alpha II included, tryptase alpha included, tryptase alpha/beta 1, Tryptase beta 1, tryptase beta I included, Tryptase I, tryptase I included, Tryptase III, Tryptase Skin) 50ul
