Tripeptidyl peptidase II (TPPII), is a large intracellular serine exopeptidase with a subtilisin active site .
TPPII protein is larger than the 26S proteasome and has a rod-shaped, dynamic supramolecular structure .
TPPII is highly conserved among species and it sequentially removes tripeptides from the free amino-terminus of short peptides.
TPPII has enhanced activity in proteosome inhibitor-adapted cells and degrades polypeptides by exo- as well as predominantly trypsin-like endoproteolytic cleavage.
TPPII participates in the apoptotic pathway triggered by Shigella and is also involved in apoptosis induced by ATP and the protein kinase inhibitor staurosporine .
Overexpression of TPPII is sufficient to prevent accumulation of polyubiquitinated proteins and partially restores peptide loading of MHC molecules .
TPPII is able to compromise proteosome activity and normalize the rate of intracellular protein breakdown, which is required for normal cellular function and viability.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of TPP2. Species Crossreactivity: Human, mouse, rat
