Glycolysis is an evolutionarily conserved series of ten chemical reactions that utilizes eleven enzymes to concomitantly generate pyruvate and ATP from glucose.
Triosephosphate isomerase, known as TIM or TPI, is ubiquitously expressed and catalyzes the interconversion of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate in the glycolitic pathway.
The human TIM gene spans 3.5 kilobase pairs, contains seven exons and encodes a 249 amino acid protein.
The TIM promoter element contains a TATA box (positions -27 to -21) and multiple GC boxes (positions -126 to -48) that variably conform to the consensus Sp1-binding site.
The GC boxes function in cis to the TATA box to control both the frequency and position of transcription initiation.
Deficiencies in TIM result in a rare autosomal recessive condition where a metabolic block in glycolysis and accumulating DHAP in erythrocytes can lead to non-spherocytic hemolytic anemia, recurrent infections, cardiomyopathy, and neuromuscular dysfunctions.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of TPI1. Species Crossreactivity: Human, mouse, rat
