The cell commits to dividing during the G2/M phase transition, and the timing of mitotic entry is tightly regulated to guarantee correct chromosome segregation.
D53, also referred to as tumor protein D52-like 1 (TPD52L1), is a coiled-coil motif-bearing member of the D52 tumor protein family.
D53 intereacts with 14-3-3, a negative regulator of the G2/M transition, in breast cancer cells.
Expression of D53 is highly upregulated at the G2/M phase transition in breast cancer cell lines, and deregulated expression of this protein can adversely affect the completion of mitosis.
D53 may function as a signaling intermediate and/or regulator of vesicle trafficking and cell proliferation.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of TPD52L1. Species Crossreactivity: Human
