84-2407-35　［取扱停止］TP53BP2 (53BP2, Apoptosis stimulating of p53 Protein 2, Apoptosis stimulating Protein of p53 2, Apoptosis-stimulating of p53 Protein 2, ASPP2, ASPP2_HUMAN, Bbp, Bcl2 Binding Protein, Bcl2-Binding Protein, NY REN 51 Antigen, NY-REN-51 Antigen, p53 Binding Protein 2, p53-Binding Protein 2, p53BP2, PPP1R13A, Renal Carcinoma Antigen NY-REN-51, TP53BP2, Tumor Protein p ...　226407

特徴

• The p53 binding proteins 53BP1 and 53BP2 (Bbp) bind to the central DNA-binding domain of wild type p53, but do not bind mutant p53.
• The central DNA-binding domain of p53 is required for site-specific DNA binding and is frequently mutated in malignant tumors.
• Binding of 53BP1 to the L3 loop of p53 and of 53BP2 to the L2 loop of p53 confirms that the loop is dependent on p53 conformation.
• Site-specific binding also suggests that 53BP1 and 53BP2 are involved in p53-mediated tumor suppression.
• 53BP1 was isolated from H258 cells and is expressed in Jurkat cells in both the cytoplasm and the nucleus.
• The N-terminus of 53BP2 is localized to the cytoplasm, while the C-terminus might be localized in the nucleus.
• 53BP1 promotes cell proliferation by binding to p202, whereas 53BP2 induces cell death by binding to Bcl2 and NFkB p65.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.