84-2407-21　［取扱停止］TOPBP1 (DNA topoisomerase 2 Binding Protein 1, DNA topoisomerase 2-Binding Protein 1, DNA topoisomerase II Binding Protein, DNA topoisomerase II Binding Protein 1, DNA topoisomerase II-beta-Binding Protein 1, DNA topoisomerase II-Binding Protein 1, DNA topoisomerase IIbeta Binding Protein 1, Hypothetical Protein KIAA0259 [Fragment], KIAA0259, TOP2BP1, TOPB1_HUMAN, ...　226398

特徴

• Human DNA topoisomerase II binding protein 1 (TopBP1) contains eight BRCT motifs that are found in proteins regulating the DNA damage response, transcription, and replication.
• In addition, TopBP1 shares sequence similarity with the fission yeast Rad4/Cut5 protein and the budding yeast DPB11 protein, both of which are required for DNA damage control and/or replication checkpoint control.
• Phosphorylation of TopBP1 occurs in response to DNA double-strand breaks and replication blocks.
• TopBP1 forms nuclear foci and localizes to the sites of DNA damage or the arrested replication forks.
• Downregulation of TopBP1 leads to reduced cell survival, probably due to increased apoptosis.
• TopBP1 functions as a transcriptional coactivator by enhancing the human papillomavirus (HPV) transcription/replication factor E2.
• In addition, the HECT-domain ubiquitin ligase, hHyD, cooperates with TopBP1 in DNA damage response.
• TopBP1 specifically interacts with the C-terminal region of topoisomerase II beta, which suggests a supportive role for TopBP1 in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.