Tollip (toll-interacting protein) serves as a suppressor of innate immunitysignaling and links the serine/threonine kinase IRAK to the IL-1 receptorcomplex upon receptor activation.
Overexpression of Tollip in HEK-293 cellsinhibits NFκB activation in response to TLR2 and TLR4 signaling.
Negativeregulation of TLR signaling by Tollip may limit the production of proinflammatorymediators during inflammation and infection.
Tollip forms a complexwith Tom1 to regulate endosomal trafficking of ubiquitinated proteins.
TheTollip protein shows ubiquitous expression in mouse.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Tollip. Species Crossreactivity: Human
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to amino acids 120-168 of Human Tollip.
