TNFalpha-IP 8 (tumor necrosis factor, alpha-induced protein 8), also known as NFκB-inducible DED-containing protein (NDED), SCC-S2 or TNF-induced protein GG2-1, is a 198 amino acid cytoplasmic protein induced by NFκB and TNF.
The induction of TNFalpha-IP 8 by TNF is dependent on the activation of NFκB.
TNFalpha-IP 8 negatively mediates apoptosis and may also play a role in tumor progression.
TNFalpha-IP 8 specifically inhibits caspase-8 activity, which results in the inhibition of BID cleavage and caspase-3 activation during TNF-mediated apoptosis.
TNFalpha-IP 8 is expressed at high levels in thymus, bone marrow, lymph node, spleen, thyroid, placenta and various tumor tissues, as well as fetal lung, liver and kidney.
TNFalpha-IP 8 is present as three isoforms produced by alternative splicing.
Applications:Suitable for use in Immunofluorescence, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of TNFalpha-IP8. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 71-116 of Human TNFα-IP83.
