Tumor necrosis factor b (TNFb), also known as lymphotoxin, is a pleiotropic cytokine.
TNFa, also known as cachectin, is a smaller cytokine that binds to the same receptors, producing a vast array of effects similar to those of TNFb.
TNFb and TNFa share 30% amino acid homology and have similar biological activities.
TNFb is produced by activated lymphocytes, including CD4(+) T helper cell type 1 lymphocytes, CD8(+) lymphocytes and certain B lymphoblastoid cell lines.
TNFa is produced by several different cell types, including lymphocytes, neutrophils and macrophages.
TNFa and TNFb can modulate many immune and inflammatory functions, while having the ability to inhibit tumor growth.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of TNF-beta. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 31-80 of Human TNF-β.
