Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice.
TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia.
TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 a band following SDS PAGE under non reducing conditions.
TNF alpha is closely related to the 25 a protein tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions.
TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity.
Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of TNF-alpha. Species Crossreactivity: Human
