The Tousled-like kinases (TLK1 and TLK2, also designated PKU-beta and PKU-alpha, respectively) are the human homologs of the Tousled gene from Arabidopsis thaliana, which encodes a Serine/Threonine kinase that is necessary for proper organ morphogenesis.
Both TLKs contain a nuclear localization signal and a predicted coiled-coil region in the N-terminal domain.
TLK is ubiquitously expressed, and is prevalent in mouse testis, especially in pachytene spermatocytes and round spermatids.
It displays a propensity to dimerize through an interaction between its coiled-coil structure and is able to autophosphorylate, as well as phosphorylate exogenous substrates.
TLK1 and TLK2 are regulated by the cell cycle, showing maximum activity during S phase.
Subsequently, they are thought to regulate the development of multicellular organisms, including playing a key role in spermatogenesis, through a series of phosphorylations.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of TLK1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human TLK1.
