84-2406-21　［取扱停止］TLE1 (E(Sp1) Homolog, Enhancer of split groucho 1, Enhancer of split groucho like Protein 1, Enhancer of split groucho-like Protein 1, ESG, Esg1, GRG1, TLE 1, Tle1, TLE1_HUMAN, Transducin like Enhancer of split 1 (E(sp1) Homolog Drosophila), Transducin like Enhancer of split 1, Transducin like Enhancer of split 1 Homolog of Drosophila E(sp1), Transducin like Enhan ...　226348

特徴

• The Notch signaling pathway controls cellular interactions important for the specification of a variety of fates in both invertebrates and vertebrates.
• Key players in the Notch pathway are the TLE genes (for transducin-like enhancer of split, also designated ESG for enhancer of split groucho), which are human homologs of the Drosophila groucho gene.
• Groucho is a transcriptional repressor that plays a key role in neurogenesis, segmentation and sex determination.
• TLEs associate with chromatin in live cells and specifically with Histone H3, but not with other core histones.
• Expression of the TLE genes, TLE1, TLE2, TLE3 and TLE4, correlate with immature epithelial cells that are progressing toward a terminally differentiated state, suggesting a role during epithelial differentiation.
• TLE1, TLE2 and TLE3 have elevated expression in cervical squamous metaplasias and carcinomas, while TLE4 is most highly expressed in the brain, particularly in the caudate nucleus.
• TLE1 and TLE4 contain SP and WD40 domains, through which TLE1 binds AML1 to inhibit AML1-induced transactivation of the CSF1 receptor.
• In early stages of cell differentiation, TLE1 is upregulated, and TLE2 and TLE4 are downregulated.
• In later stages, TLE2 and TLE4 are upregulated, and expression of TLE1 decreases.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.