TK2 phosphorylates these nucleosides to their corresponding nucleoside monophosphates using a nucleotide triphosphate as a donor.
Deficiency of mitochondrial TK2 manifests as severe skeletal myopathy during infancy, due to depletion of mtDNA.
Mutant enzyme possesses similar K(m) values to wild type, however, the V(max) is markedly decreased, leading to the decreased enzyme efficiency, which causes the disease.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of TK. Species Crossreactivity: Human, mouse, rat
