Mammalian telomeric proteins consist of TRF1 (telomeric repeat binding factor), TRF2, tankyrase, and TIN2, which have no recognized orthologs in the budding yeast, Saccharomyces cerevisiae, and RAP1, which is an ortholog to the yeast telomeric protein scRap1.
Like scRap1, mammalian RAP1 regulates telomere elongation.
RAP1 interacts with two proteins, Rif1 and Rif2, which contribute to telomere length homeostasis.
Unlike scRap1, which binds telomeric DNA directly, RAP1 is recruited to telomeres by TRF2.
The functional and structural similarities of scRap1 to mammalian RAP1 suggest that the budding yeast preserved RAP1 at telomeres, but lost the TRF component.
The telomeric protein TRF1 requires TIN2 to control telomere length in human cells.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of TIN2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 60-110 of Human TIN2.
