TIEG1 and EGR-alpha are expressedfrom alternate promoters of the same gene.
TIEG1 and EGR-alpha are both highly expressed in human fetal osteoblast cells.
TIEG1 is additionally expressed at high levels in PBLs, spleen and colon, and at lower levels in thymus, small intestine, ovary, prostate and skeletal muscle.
The nuclear TIEG2 protein, which shares significant homology with TIEG1, was originally isolated from globin-expressing human fetal eryth-roid cells.
TIEG2 is also expressed in fetal liver.
Overexpression of TIEG2 in cultured epithelial cells inhibits cellular proliferation; TIEG2 expression is upregulated by TGFbeta1 and serum deprivation.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of TIEG2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 1-50 of Human TIEG2.
