84-2405-36　［取扱停止］TGM3 (E Polypeptide Protein glutamine gamma glutamyltransferase, Protein glutamine gamma glutamyltransferase E 27kD Catalytic Chain, Protein glutamine gamma glutamyltransferase E 50kD nonCatalytic Chain, Protein glutamine gamma glutamyltransferase E, Protein-glutamine gamma-glutamyltransferase E 27kD non-Catalytic Chain, TG(E), TGase E, TGase-3, TGE, TGM 3, TGM3, ...　226305

特徴

• TGases (transglutaminases) catalyze the post-translational modification of proteins by transamidation of available glutamine residues, resulting in a stable, insoluble macromolecular structure.
• The human haploid genome contains at least five distinct transglutaminases that are differentially expressed in time-space and tissue-specific ways.
• Epidermal-type TGase (TGase3), also known as TGE, TGX and TGM3, is involved in the formation of the cornified cell envelope by cross-linking a variety of structural proteins in the epidermis.
• It is expressed during late stages of terminal differentiation of the epidermis and in certain cell types of the hair follicle.
• TGase3 is widely expressed and is important for epithelial barrier formation.
• It is a zymogen, requiring proteolysis for activity.
• TGase3 is devoid of GTPase activity, but its TGase activity is inhibited by GTP as in the case of tissue-type TGase (TGase2).
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.