Transforming growth factor-alpha (TGFalpha) is an acid- and heat-stable 50 amino acid protein originally found in rodents and humans.
TGFalpha is 33% homologous at the amino acid level to epidermal growth factor (EGF).
TGFalpha binds to the EGF receptor, mediates tyrosine phosphorylation of the receptor and promotes anchorage-independent growth of normal rat fibroblasts in soft agar in the presence of transforming growth factor-beta.
TGFa is secreted by a variety of transformed cells and tumors, embryonic cells and some normal adult cells.
TGFalpha bioactivity has been found in the urine of cancer patients.
It has been suggested that it may act as an autocrine growth factor for the induction or maintenance of malignancy
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of TGFalpha. Species Crossreactivity: Human, mouse, rat
