TG1019 is a G protein-coupled receptor that functions as a chemoattractant for eosinophils and neutrophils.
TG1019 is expressed in various tissues except the brain, with the highest expression in liver, kidney, peripheral leukocyte, lung and spleen.
TG1019 interacts with the protein OXE to form TG1019/OXE, a Galpha i/o protein-coupled receptor.
Signals from TG1019 are transduced via a Galpha i/o protein to PLC/calcium, MEK/ERK and PI3K/Akt pathways.
Signal transduction from TG1019 following stimulation with 5-oxo-6E,8Z,11Z,14Z eicosatetraenoic acid (5-oxo-ETE) induces intracellular calcium mobilization and rapid activation of MEK/ERK and PI3K/Akt pathways.
TG1019 also may play a role in downregulating cAMP production.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of TG1019. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 220-270 of Human TG1019.
