A member of the TEA/ATTS domain family, Transcriptional enhancer factor 1 (TEF-1) is a nuclear protein that is expressed in numerous cell types and plays a role in controlling the expression of numerous genes.
TEF family members have a highly conserved DNA-binding domain; TEF-1 binds to GT-IIC, SphI/II and M-CAT.
TEF-1 also binds to the proximal regulatory element (PRE) of transforming growth factor-alpha, a member of the EGF family that is overexpressed in many types of cancer.
Furthermore, TEF-1 represses transcription in placental cells.
In vitro, TEF-1 is phosphorylated by several PKC isozymes.
TEF-1 is phosphorylated in vivo at serine and threonine residues.
Phosphorylation of TEF-1, both in vivo and in vitro, results in a reduction in its DNA-binding capability, which suggests a potential role for TEF-1 in PKC inhibition.
TEF-1 also complexes with larger tumor antigen (TAg), and may thus have a role in tumorigenesis.
Dimerization of TEF-1 may be important for TEF-1 to function as a regulator of gene transcription.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunocytochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of TEF-1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 105-150 of Human TEF-1.
