Cytotoxic T lymphocyte (CTL)-mediated cytotoxicity constitutes an important component of specific effector mechanisms in immunosurveillance against virus-infected or -transformed cells.
Two mechanisms appear to account for this activity, one of which is the perforin-based process.
Independently, a FAS-based mechanism involves the transducing molecule FAS (APO-1) and its ligand (FAS-L).
The human FAS (APO-1) protein is a 48kD cell surface glycoprotein that belongs to a family of receptors that includes CD40, nerve growth factor receptors and tumor necrosis factor receptors.
The FAS antigen is expressed on a broad range of lymphoid cell lines, and is expressed at high levels in T cells subsequent to crosslinking of the T cell receptor (TCR).
A previously undescribed protein, TDAG51, restores activation- induced apoptosis in cells that have lost the ability to display Fas in response to activation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of TDAG51. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 120-165 of Human TDAG51.
