TCFL5 [transcription factor-like 5 (basic helix-loop-helix)], also known as cha transcription factor, HPV-16 E2-binding protein 1, CHA, Figlb, E2BP-1 or MGC46135, is a 500 amino acid protein belonging to the basic helix-loop-helix (bHLH) family.
TCFL5 localizes to the nucleus and may function as a transcription factor during early spermatogenesis.
TCFL5 is known to form a complex with upstream stimulatory factor (USF)-1, thereby negatively regulating USF-dependent transcription.
Six isoforms of TCFL5 exist due to alternative splicing; isoform 2 is pancreas specific and isoform 3 is testis specific showing expression in primary spermatocytes at the pachytene stage.
Antibodies against TCFL5 have been identified in patients with Chagas' disease, which is caused by Trypanosoma cruzi infection.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of TCFL5. Species Crossreactivity: Human, mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to amino acids 260-311 of Human TCFL5.
