T-cell factor-1 (TCF-1) is a DNA-binding transcriptional activator that isessential for lymphoid cell development.
The TCF family of transcription factors are activated by the Wnt-1 and Wingless pathways and are characterized by the presence of a conserved protein motif, the high mobility group (HMG) 1 box, which mediates DNA binding.
Several alternative splice variants of TCF-1 have been identified, including TCF-1A, which share a conserved amino terminus and differ in the carboxy terminal sequences.
The Wnt mediated signaling pathway induces cytosolic beta-catenin binding to TCF proteins within the nucleus, leading to the enhanced expression of the Wnt target genes.
The beta-catenin-TCF complexes are negatively regulated by the adenomatous polyposis coli (APC) tumor suppressor protein, which phosphorylates beta-catenin and, in turn, increases the degradation of cytosolic beta-catenin and inhibits the transcriptional activity of the TCF proteins.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of TCF-1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids N-terminus of Human TCF-1.
