The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box.
This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters.
TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex.
As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene.
However, it is estimated that only 10-20% of human promoters have TATA boxes.
Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II.
This protein is not suitable for samples where the nuclear envelope has been removed.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of TBP. Species Crossreactivity: Human, Mouse, Rat
