The transcriptional co-activator with PDZ-binding motif (TAZ) is a 14-3-3-binding molecule.
The highly conserved and ubiquitously expressed 14-3-3 proteins regulate differentiation, cell cycle progression and apoptosis by binding intracellular phosphoproteins involved in signal transduction.
TAZ may link events at the plasma membrane and cytosketeton to nuclear transcription in a manner that can be regulated by 14-3-3.
TAZ shares homology with the WW domain of yes-associated protein (yAP) and functions as a transcriptional co-activator by binding to the PPXy motif present on transcription factors.
TAZ recognizes immunoreactive protein bands in lysates from MDCK, NIH-3T3 and 293T cells.
In addition, COS7, Hep G2, CHO and HeLa cells express endogenous TAZ.
14-3-3 binding requires TAZ phosphorylation on a single Serine 89 residue, resulting in the inhibition of TAZ transcriptional co-activation through 14-3-3-mediated nuclear export.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of TAZ. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 167-212 of Human TAZ.
